Diagnostic value of HLA-B27 by flow cytometer in ankylosing spondylitis
- VernacularTitle:流式细胞仪检测人类白细胞抗原B27对强直性脊柱炎的诊断价值
- Author:
Qingrui YANG
;
Yuanchao ZHANG
;
Xueqiang FANG
- Publication Type:Journal Article
- Keywords:
Spondylitis,ankylosing;
HLA-B27;
Flow cytometer assay;
Diagnostic tests
- From:
Chinese Journal of Practical Internal Medicine
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the diagnostic value of HLA-B27 in ankylosing spondylitis(AS).Methods A diagnostic test was employed in the study.The patients with ankylosing spondylitis and the controls in Shandong Provicial Hospital from 2001—2005 were enrolled in the study.HLA-B27 was detected by flow cytometer assay when recruited.SPSS 13.0 was used for statistical analysis.Results Totally 133 patients with AS and 88 controls were available in the study.HLA-B27 was significantly different between AS group and the controls(P=0.000).The areas below ROC curve was 90.5%.Three cut-off points of HLA-B27,11.55%,46.6% and 85.25%,were selected based on clinical practice and ROCcurve,and the sensitivity was 94.7%,90.2% and 63.6%,the specificity was 56.8%,92.0% and 95.45%.Conclusion HLA-B27 which is more than 46.6% is a valuable marker for the diagnosis of AS.
