Nerve cells injury in hippocampal region after focal cerebral ischemia-reperfusion for rats with diabetes
- VernacularTitle:糖尿病大鼠局灶性脑缺血再灌注后海马区神经元损伤的观察
- Author:
Xin DING
;
Jisheng ZHANG
;
Wei LAN
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Cerebral ischemia-reperfusion;
Hippocampus;
Apoptotic neurons
- From:
Chinese Journal of Practical Internal Medicine
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the effect of diabetes on the nerve cells injury in hippocampal region by observing the nerve cells changes in this region of ischemia after focal cerebral ischemia reperfusion for rats with diabetes.Methods Sixty healthy male Wister rats were randomly divided into four groups:the control group,sham operation group,normal cerebral ischemia-reperfusion(NIR)group,and diabetic cerebral ischemia-reperfusion(DIR)group.The model of local cerebral ischemia and reperfusion was established by the thread-embolism and cerclage of rats with STZ-induced diabetes.Hematoxylin-eosin(HE)-stained neurons in hippocampal CA1 region were observed by selecting 8 un-overlapped fields under light microscope(?400);the total number of the normal pyramidal neurons with whole cellular organ structure were counted within 100 ?m length in CA1 region.The numbers of positive neurons were presented as mean with standard errors(?s).Significant differences between groups were analyzed by t-test.Results Neuronal loss or apoptotic cells were not found in the control group or sham operation group after observing under light microscope.There were neuronal losses in both NIR and DIR groups,neuronal loss of DIR group being more serious than that of NIR group(P
