Effect of alcoholic extract from Fructus Polygoni Orientalis on lipid peroxidation in rat tissues
- VernacularTitle:水红花子醇提物抑制大鼠组织脂质过氧化反应的体外作用研究
- Author:
Bin GE
;
Zhenming ZHANG
;
Aixia XU
;
Xiang GAO
;
Xiaoyan LEI
- Publication Type:Journal Article
- Keywords:
alcoholic extract from Fructus Polygoni Orientalis;
malondialdehyde;
free radicals;
hemolysis;
erythrocyte;
neutrophil;
antioxidation
- From:Journal of Third Military Medical University
1988;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the activities of the alcoholic extract from Fructus Polygoni Orientalis (EFPO) against antioxidation. Methods The peroxidation in homogenate of rat heart, liver and kidney was induced by ?OH generation system Fe2++ascorbic acid. Malondialdehyde (MDA) was determined by TBA colorimetric method. Superoxide (O-2) from zymosan-stimulated neutrophils of rat was measured by NBT reduction method. H2O2-caused hemolysis of erythrocytes was measured by spectrometry. Results MDA in homogenate of heart, liver and kidney of rat that induced by ?OH generation system Fe2++ascorbic acid was inhibited by 6.3, 12.5, 25, 50, 100, 200 ?g/L EFPO; IC50 of EFPO to MDA in heart, liver and kidney was 31.8, 32.5 and 40.2 ?g/L respectively and its dose-effect relation was of negative relativity and the relative coefficient was -0.886, -0.874 and -0.918 (all P
