Comparative proteomic analysis of differently differentiated human laryngeal squamous cell cancer
- VernacularTitle:不同分化喉鳞状细胞癌蛋白质组差异表达的研究
- Author:
Jianrong ZHOU
;
Lianzhi WEI
;
Yinping LI
;
Zhongxue FU
- Publication Type:Journal Article
- Keywords:
human laryngeal squamous cell cancer;
protein;
two-dimensional polyacrylamide gel electrophoresis
- From:Journal of Third Military Medical University
1988;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish two-dimensional electrophoresis profiles of laryngeal squamous carcinoma tissue with high resolution and reproducibility. Methods The total protein of well-differentiated or poorly-differentiated laryngeal cancer tissue was separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE). The differential expression protein was analyzed by using ImageMaster 2D analysis software. Results We obtained fine resolution, reproducible 2-DE patterns of well-differentiated and poorly-differentiated laryngeal squamous carcinoma. Differential expression protein spots were defined in 2-DE gels. Thirteen protein spots were preliminarily identified, among which seven proteins were upregulated in well-differentiated laryngeal cancer tissue, six were downregulated. Conclusion The expression map of proteomics was established for well-differentiated and poorly-differentiated laryngeal cancer, which was of significant difference between them. Laryngeal cancer proteome might reflect the underlying pathological state.
