Determination of Serum Tryptophan and Kynurenine in Pregnant Women
- VernacularTitle:高效液相色谱法测定孕妇血清中色氨酸及其代谢产物Kynurenine
- Author:
Xuemei XU
;
Xiaoping XIA
- Publication Type:Journal Article
- Keywords:
HPLC;
Trp;
Kynurenine;
pregnant measurement
- From:
Journal of Zhejiang Chinese Medical University
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To establish a method for determination of serum tryptophan and kynurenine in pregnant women.[Method] RP-HPLC was applied to analysis.C18 column was used.The mobile phase was composed of methanol-0.001M KH2PO4(PH=4)(12∶88).The UV detection wave length was 245nm.[Results] The linear response ranged from 2.56～307.06?mol?L-1 for tryptophan, r=0.9999.The linear response ranged from 0.12～5.05?mol?L-1 for kynurenine, r=0.9997.The mean concentrations of serum tryptophan and kynurenine were 49.73?7.48?mol?L-1 and 0.74?0.33?mol?L-1 respectively in 30 pregnant women.[Conclusion] The method is sensitive and suitable for the determination.
