Effect of atorvastatin on the expression of inhibitor of nuclear factor I?B? induced by lipopolysacchairde in human vascular endotheliar cells
- VernacularTitle:阿托伐他汀对LPS诱导人血管内皮细胞I?B?表达的影响
- Author:
Gang ZHAO
;
Guinan LIU
;
Zhiming LI
- Publication Type:Journal Article
- Keywords:
atorvastatin;
inhibitor of nuclear factor ?B?;
phosphorylated inhibitor of nuclear factor ?B?
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect and mechanism of atorvastatin on lipopolysacchairde (LPS) inducing the expression of inhibitor of nuclear factor ?B? (I?B?) in human vascular endotheliar cells. Methods The human vascular endotheliar cell line ECV304 was cultured and divided into five groups as control group, LPS group, and low, moderate or high does atorvastatin groups. After incubated with different densities atorvastatin, the three atorvastatin groups and LPS group were stimulated with LPS 30min. Then the activation of I?B? was observed with immnofluorescence. The proteins expressions of I?B? and phosphorylated I?B? were detected with western blot. The ex-pression of I?B? mRNA was examined with reversetranscription-polymerase chain reaction. Results Atorvastatin could inhibit the translocation of p65 to the nucleus and reduce the phosphorylation and degradation of I?B? in a dose-dependent manner. The high density atorvastatin could increase the expression of I?B? mRNA. Conclusion The atorvastatin can inhibit the activation of nuclear factor ?B by regulating the expression and degradation of I?B?.
