Calcium phosphate cement with rhVEGF and rhBMP-2 promotes revascularization of big section bone allograft in dogs
- VernacularTitle:钙磷骨水泥复合rhVEGF与rhBMP-2促进异体大段骨移植再血管化的实验研究
- Author:
Xiangyang QU
;
Dianming JIANG
;
Hong AN
;
Yong LIANG
- Publication Type:Journal Article
- Keywords:
calcium phosphate cement;
deproteinized bone;
vascular endothelial growth factor;
bone morphogenetic protein;
bone allograft
- From:Journal of Third Military Medical University
1988;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the ability of revascularization of big section deproteinized bone (DPB) allograft combining calcium phosphate cement (CPC) with recombinant human vascular endothelial growth factor (rhVEGF) and bone morphogenetic protein-2 (rhBMP-2). Methods Totally 36 adult hybrid dogs were inflicted to demi-articular defect models by exsecting 30 mm right femur inferior extremity near articular surface,and then randomly divided into 3 groups, group A, receiving material CPC/rhVEGF/rhBMP-2/DPB transplantation into the demi-articular defect, group B, material rhVEGF/rhBMP-2/DPB transplantation, and group C with simple material DPB transplantation. At 4, 8, 12 and 16 weeks after operation, vasculogenesis and new bone formation were assessed by X-ray examination, histological examination, transmission electron microscopy, vas capillare analysis by vaso-filling with ink and radionuclide bone imaging examination (ECT). Results The bone formation and vascularization was significantly superior in group A to that in group B and C. The results of vaso-filling examination with ink and arterial perfusion of ECT examination were both significantly superior to that in group B and C (P
