Inhibitory effect of macrophage colony stimulating factor on lung cancer cell line A549
- VernacularTitle:巨噬细胞集落刺激因子对肺癌细胞株A549在体外的抑制作用
- Author:
Shengzu PENG
;
Jiaxin MIN
;
Li JIANG
- Publication Type:Journal Article
- Keywords:
macrophage colony stimulating factor;
lung cancer;
growth inhibition;
cell cycle;
macrophage colony stimulating factor receptor
- From:Journal of Third Military Medical University
1988;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of macrophage colony stimulating factor (M-CSF) on lung cancer cell line A549. Methods After M-CSF at 0.1, 1 and 10 ng/ml was given to cultured A549 cells for 0, 24, 48, 72 and 92 h respectively, their morphological changes were observed with inverted microscopy, proliferation was measured by MTT assay, and cell cycles were determined by flow cytometry. RT-PCR was used to detect the change of expression of M-CSF receptor. Result The growth of A549 was significantly inhibited by M-CSF in a concentration- and time- dependent manner. The maximum response was obtained at 10 ng/ml of M-CSF. Flow cytometric analysis revealed that the treated A549 cells arrested at the G0/G1 phase of the cell cycle. RT-PCR showed that the mRNA expression of M-CSF receptor was reduced after the M-CSF treatment. Conclusion M-CSF has an anti-tumour activity on lung cancer cell A549.
