Experimental studies on calcium ion concentration and mitochondrial membrane potential in cultured myocyte of rat subjected to hypovolemia shock
- VernacularTitle:低血容量性休克大鼠心肌细胞Ca~(2+)浓度及膜电位的变化
- Author:
Qingshan TENG
;
Ao FEI
;
Yumei ZHANG
- Publication Type:Journal Article
- Keywords:
hypertonic solutions;
shock;
myocardium;
cell culture;
electrophysiology;
calcium concentration;
membrane potentials
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes in Ca2+ concentration and mitochondrial membrane potential in cultured myocytes in rat subjected to hypovolemia shock.Methods 84 Wistar rats were randomized into two groups:hypertonic solution resuscitation(HES,n=42)and 0.9% sodium chloride resuscitation(NS,n=42).At different timepoint(pre-shock stage,shock,post-resuscitation 5min,15min,30min,60min and 90min),the cultured cardiac myocytes,some were stained with JC-1,used fluo-4/AM as calcium probe,the others were assayed for Ca2+ mitochondrial membrane potential by flow cytomety.Results Ca2+ concentration of group HES at shock,post-resuscitation 5min,15min and 30min was higher than that of pre-shock stage,but mitochondrial membrane potential at post-resuscitation 60min and 90min was lower(P0.05).Ca2+ concentration and mitochondrial membrane potential of group HES at post-resuscitation 60min and 90min were more significantly different than that of group NS(P
