Proteomic analysis of lymph nodes affected by esophageal squamous cell carcinoma by two-dimensional gel electrophoresis
- VernacularTitle:食管鳞癌转移淋巴结蛋白质双向凝胶电泳图谱差异分析
- Author:
Bin WANG
;
Juncai LI
;
Zhongxue FU
;
Da ZHU
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma;
lymphatic metastasis;
proteome;
two-dimensional gel electrophoresis
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the differential expression of proteome between metastatic lymph nodes and normal lymph nodes in esophageal squamous cell carcinoma.Methods Two dimensional polyacrylamide gel electrophoresis(2-DE)technique and computer-assisted image analysis were used to separate the protein spots and analyze the differential expression of proteome in the metastatic lymph nodes and normal lymph nodes.Results There were 23 protein spots significantly differentially expressed,of which 4 protein spots were newly found,1 protein spot disappeared,14 protein spots increased markedly,and 4 protein spots decreased significantly.Conclusion The proteomic expression in metastatic lymph nodes of esophageal squamous cell carcinoma is significantly different from that of the normal lymph nodes,which may be associated with the mechanism of metastasis of esophageal squamous cell carcinoma.
