ANTAGONISM OF SELENIUM ENRICHED SPIRULINA PLATENSIS TO HEPATOCIRRHOSIS AND ITS MECHANISM
- VernacularTitle:硒螺旋藻拮抗大鼠肝纤维化的作用及机制研究
- Author:
Zhi HUANG
;
Wenjie ZHENG
- Publication Type:Journal Article
- Keywords:
Spirulina platensis;
selenium;
hepatocirrhosis;
selenoenzyme;
proliferation
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the antagonism of selenium enriched Spirulina platensis(Se-SP) to hepatocirrhosis by enhancement of cell proliferation and selenoenzyme activity.Method: The male SD rat hepatocirrhosis model was induced by thioacetamide(TAA),and Se-SP was supplemented.Hepatic histological analysis was performed and relative content of collagen(RCC %) was estimated using IBAS 2000 system after Masson’s staining.Glutathione peroxidase(GSH-Px),thioredoxin reductases(TR) and malondialdehyde(MDA) in hepatocyte as well as hyaluronic acid(HA) in serum were determined.Expression index of proliferating cell nuclear antigen(PCNA) in hepatocytes was detected by immu-nohistochemistry,and the level of synthesis of DNA in regenerative hepatocytes was analyzed by radio-immunity for incorporation of 3H-TDR.Results: Hepatocirrhosis was induced by TAA at 9w of the expeiment,and the obvious antagonism of Se-SP to hepatocirrhosis was obsewed after Se-SP supplementa-tion.12.5% rats of Se-Sp group were completely recovered at 15w of the experiment(6w after withdrawal of TAA).GSH-Px and TR activity in hepatocytes as well as PCNA and 3H-TDR incorporation rate in hepatocytes were obviously enhanced(P
