THE ANTIOXIDATIVE ACTIVITY AND ANTI-PROLIFERATION ACTIVITY OF PURIFIED TEA POLYSACCHARIDE AGAINST TWO COLON CANCER CELL LINES
- VernacularTitle:纯化茶多糖的抗氧化活性及其对两种结肠癌细胞增殖抑制作用
- Author:
Shaoping NIE
;
Mingyong XIE
;
Shuwen CAO
- Publication Type:Journal Article
- Keywords:
tea polysaccharide;
antioxidant activity;
colon cancer cells;
anti-proliferation
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the antioxidant activity and anticancer activity of tea polysaccharide.Methods:Tea polysaccharide was extracted and purified by gel permeation chromatography from coarse old green tea leaves obtained from Wuyuan county of Jiangxi province in China.The antioxidant activity of the tea polysaccharide was evaluated by determining the change in absorbancy after heat-induced oxidation in a linoleic acid system with ?-carotene,the decoloration of the 1,1-diphenyl-2-picrylhydrazyl and the elimination of superoxide generated from autoxidation of pyrogallol.On the other hand,the anticancer activities of tea polysaccharide at different concentrations were evaluated by MTT assay using two colon cancer cell lines(HCT-15,Caco-2).Results:Tea polysaccharide possessed distinctive antioxidative activity.The anti-proliferation capabilities of the tea polysaccharide against the tested human colon cancer cell lines were different under the designed experiment conditions.The tea polysaccharide with high purity exhibited good anti-proliferation activity against HCT-15 at concentration of 7.0?mol/L.Conclusion : Tea polysaccharide has remarkable antioxidative and anticancer bioactivities..
