Research into the standard process of fresh freeze - dried plasma for providing medical treatments with dutch dryer LY3-TTE/DM8
- Author:
Thin Duy Ngo
;
Binh -- Tran
;
Duong Tuan Pham
;
Phan Trung Do
- Publication Type:Journal Article
- Keywords:
Dryer LY3-TTE/DM8-Fresh-Plasma-drying process stem cell
- MeSH:
Plasma
- From:Journal of Medical Research
2007;49(3):65-68
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Background: Freeze - Dried Plasma (FDP) has many advantages, which could be stored for a long \ufffd?term and transported across remote areas for providing medical treatments and emergency aid. Objectives: To study the standard process of freeze - dried fresh plasma. Subjects and methods: 10 samples of fresh plasma from volunteer donors were selected according to certain categories. Plasma samples were frozen at \ufffd?75oC in 12 hours, freeze - dried at - 45oC and at the atmospheric pressure of 0.04mbar with Dutch dryer Ly - TTE/DM8. The adequate freeze - drying time for every 100ml of plasma was 60 hours. The protein level, factor VIII, electrolysis, the pH of plasma before and after the drying process were evaluated. Results: Results of tracking protein level in comparison between before and after freeze - dry showed the components and levels of freeze-dried protein changed little, with no statistical significance (P>0.05). The clotting factor before and after freeze-dried retains standard activity. After the freeze-dried process, factor VIII achieved 0.80 Ul/ml, prothombin achieved >80%. However, prothrombin decreased clearly (P<0.05). Electrolytes and pH changed had no statistical significance (P> 0.05). Conclusions: The procedures are applicable for processing and manufacturing drying plasma for long-term-storage and medical treatment. \r\n', u'\r\n', u'\r\n', u'
