Immunomodulative effect of hesperidin on immunodepressed mice
- VernacularTitle:橙皮苷对免疫功能低下小鼠免疫调节作用的实验研究
- Author:
Rong LI
;
Jun LI
;
Chengmu HU
;
Lei ZHANG
;
Qian ZHANG
- Publication Type:Journal Article
- Keywords:
hesperidin;
immunoloregulation effect;
hemolysin;
nonspecific immunity;
humoral immunity;
cellular immunity
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the immunomodulative effect of hesperidin on immunodepressed mice.Methods The immunosuppressed mice were induced by cyclophosphamide (Cy, ip). Indexes of immune organs were calculated. Phagocytosis of mononuclear macrophage was determined by cleaning carbon particle method. Spectrophotography was used to estimate levels of serum specific IgG, IgM (HCIgM, HCIgG). Plaque forming cell (PFC) was determined with quantitative haemolysis of SRBC (QHS). Splenic lymphocytes proliferation was measured by MTT method. The mouse delayed type hypersensitivity (DTH) model induced by dinitoflruorobenzene (DNFB) was used to study the effect of hesperidin on the level of DTH and subset of T lymphocyte. Results Hesperidin remarkably increased indexes of spleen and thymus, the rate of clearance and clearance index, but had no significant impact on HCIgM, HCIgG and PFC. In addition, it could enhance the proliferation of splenic lymphocytes and reverse DTH response to normal level. Conclusion Our results indicated that hesperidin had an enhanced effect on nonspecific immunity and specific cellular immunity in immunodepressed mice, while specific humoral immunity wasn′t significantly changed.
