Effect of 15d-PGJ_2 on proliferation and apoptosis of HSC-T6
- VernacularTitle:15d-PGJ_2对肝星状细胞增殖和凋亡的影响
- Author:
Yi KANG
;
Tiancai WANG
;
Xiuling LI
;
Yuxiu YANG
;
Jia SHANG
- Publication Type:Journal Article
- Keywords:
15d-PGJ2;
PPAR?;
HSC;
apoptosis;
NF-?B
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To evaluate the effect of 15d-PGJ2 on up-regulated expression of PPAR? and inducing HSC apoptosis and inhibiting HSC proliferation. Methods The rat liver stellate cell line (HSC-T6) was cultured in DMEM, and treated with PPAR? agonist 15d-PGJ2 of different concentrations. The expression of PPAR? mRNA was detected by RT-PCR. The protein expression of NF-?B was examined by Western blot. The cell proliferation rate of HSC-T6 was determined by MTT colorimetric assay. The cell cycle and apoptosis ratio were measured using flow cyto -metry analysis. Results The proliferation rate of the rat liver stellate cell line (HSC-T6) was significantly inhibited by 15d-PGJ2 (vs controls, P
