Effects of cinnamic acid on differentiation of MGC-803 cells
- VernacularTitle:肉桂酸对胃腺癌细胞诱导分化的实验研究
- Author:
Juan LU
;
Hui WANG
;
Fangan LU
- Publication Type:Journal Article
- Keywords:
cinnamic acid;
gastric carcinoma cell;
induced differentiation
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate effects of cinnamic acid (CINN) on the differentiation of MGC-803 cells. Methods MGC-803 cells were treated by CINN (1, 2 and 3 mmol?L-1), and the rate of growth suppression was evaluated by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Plate clone formation assay was carried out to detect the phenotypes of colony formation. FACS analysis was used to analyze the cell cycle. The activity of telomerase was examined by telomeric repeat amplification protocol (TRAP) PCR-silver staining. Results CINN showed significant inhibitory effect on growth of these gastric carcinoma cells at 24, 48, 72 and 96 h. Treated with CINN for 48 h, cell cycle showed G0/G1 phase arrest, and the activity of telomerase was inhibited significantly. The colony-forming rate was reduced drastically (P
