Hypoxia induces the expression and secretion of connective tissue growth factor and fibronectin by cultured renal cortical myofibroblasts
- VernacularTitle:低氧促进肌成纤维细胞表达和分泌结缔组织生长因子及纤维连接蛋白
- Author:
Liping GUO
;
Haichang HUANG
;
Jingzi LI
- Publication Type:Journal Article
- Keywords:
Hypoxia;
Myofibroblasts;
Fibronectins;
Connective tissue;
Growth Substances
- From:
Journal of Peking University(Health Sciences)
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate whether hypoxia can affect the expression and secretion of connective tissue growth factor(CTGF) and fibronectin(FN) in primary cultured rat renal cortical myofibroblasts . Methods: The primary cultured rat renal cortical myofibroblasts were subjected to hypoxic (1%O_2) or normoxic (21% O_2) conditions for a variety of times. The protein levels of HIF - 1?, CTGF and FN protein were analyzed by Western blotting in both the whole cell lysates and supernatant culture medium 6 h,12 h and 24 h after incubation, respectively. RT-PCR was carried out to measure the levels of FN mRNA at different time points (2 h,3 h,6 h and 12 h). The activity of gelatinase MMP-2 and MMP-9 in the supernatant from the cultured cell medium was assayed by gelatin zymography. Results: The expression of HIF - 1?was induced at h6 in cells under hypoxia incubation. The levels of cellular CTGF protein were increased in hypoxia treated myofibroblasts at h6 (175%?52%),significantly elevated at h12 (347%?67%,P
