Real-time quantification of microRNAs by RNA-tailing and primer-extension RT-PCR
- VernacularTitle:RNA加尾和引物延伸RT-PCR法实时定量检测microRNA
- Author:
Qi ZHANG
;
Xiangjun HE
;
Xiuying PAN
- Publication Type:Journal Article
- Keywords:
MicroRNAs;
Reverse transcriptase polymerase chain reaction;
Laboratory techniques and procedures
- From:
Journal of Peking University(Health Sciences)
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize and evaluate the modified RNA-tailing and primer-extension RT-PCR method in relative quantification of microRNAs (miRNAs) in several kinds of tissues. Methods: Small-sized RNAs (
