Antibody array analysis of atherosclerosis cytokine levels in rats

VernacularTitle:大鼠动脉粥样硬化病变组织致炎-抗炎因子抗体芯片分析
Author: Jishan FAN ; Xiaohui LI ; Shuhui LI ; Zhibing YUAN
Publication Type:Journal Article
Keywords: atherosclerosis, cytokines, antibody array
From:Journal of Third Military Medical University 1983;0(03):-
CountryChina
Language:Chinese
Abstract: Objective To investigate the changes of the cytokines on the aortas of atherosclerosis (AS) rats. Methods Zymosan-liquid medical paraffin suspension (contain Zymosan 20 mg/kg) was injected intra- peritoneally once a week for 10 times and given intraperitoneal injections of ovalbumin, 2.5 mg/kg, once a week for 7 times after initial subcutaneous sensitization. All animals were fed cholesterol-rich diet to induce AS. Electron microscopy was used to observe the foam cells, and RayBio Rat Cytokine Antibody Array 1.1 was employed to detect the cytokines in the aorta wall. Results Electron microscopy showed all models had monocytes migration from lumen to subendothelium in the aorta and formed to foam cell for phagocytosis lipid, indicating typical characteristic of AS in the rats. Most pro-inflammatory cytokines were increased except Fractalkine and IL-6, but anti-inflammatory cytokines were all slightly decreased. Conclusion Inflammation and immune can induce the formation of foam cells. The balance of pro-inflammatory cytokines and anti-inflammatory cytokines is broken is the important reasons of atherogenesis。