Apoptosis of endometrial adenocarcinoma cell line JEC induced by influenza virus H_3N_2
- VernacularTitle:甲型流感病毒H_3N_2诱导人子宫内膜腺癌细胞凋亡的实验研究
- Author:
Huan WANG
;
Zhongming WU
;
Rui MA
;
Yu WANG
- Publication Type:Journal Article
- Keywords:
influenza virus A;
apoptosis;
endometrial adenocarcinoma cells
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the apoptosis-inducing effects and mechanisms of influenza virus A H_ 3 N_ 2 on a self-established endometrial adenocarcinoma cell line JEC. Methods JEC cells were infected with different concentrations of H_3N_2, the apoptosis were detected using the HE staining, DNA agarose gel electrophoresis and flow cytometry. The expressions of Fas, FasL and TGF-? were examined by immunocytochemical staining. Results After infection, the JEC cells showed the morphological apoptosis; DNA agarose electrophoresis demonstrated a ladder-like pattern of DNA fragments; FITC/PI stained FCM showed the apoptotic rate of JEC had been decreased along with the prolonging of infected time and elevated with the increase of the virus concentrations. Immunocytochemical staining showed that enhanced expression of Fas, attenuated expression of TGF-?, and no expression of FasL after H_3N_2 infection. Conclusion Influenza virus A H_3N_2 can induce JEC cells to apoptosis in time- and concentration- dependent manners, which may be related with the expressions of Fas and TGF-?.
