Study on NGF expression and phenotype transformation of AMCC in asthmatic rats
- VernacularTitle:哮喘大鼠肾上腺髓质嗜铬细胞表型转化与神经生长因子表达水平初探
- Author:
Jun WANG
;
Chengping HU
;
Weijun LIANG
- Publication Type:Journal Article
- Keywords:
Asthma;
AMCC;
NGF;
Adrenaline
- From:
Chinese Journal of Practical Internal Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of nerve growth factor(NGF)in the adrenaline medullary chromaffin cells(AMCC)and the morphological and functional changes in AMCC of asthmatic rats.Methods The 32 SD rats were randomly divided into two groups:control group(n=8)and asthma group(n=8).Asthma models were established by sensitization and challenge with ovalbumin(OVA).By means of immunohistochemistry(SP)combined with the micro-image analysis to investigate the alterations of NGF immunoreactivity in asthmatic rats and by means of light microscopy and electron microscopy to investigate the ultrastructural changes in AMCC,and detect the concentration of adrenaline and noradrenaline in serum by enzyme-linked- immunosorbent assay(ELISA).Results The positive immunoreactivity was increased in asthmatic AMCC compared with the controls(P
