Effects of celecoxib on the expression of PGE2 and VEGF A549 cell lines
- VernacularTitle:塞来昔布对肺癌A549细胞前列腺素E_2和血管内皮生长因子表达的影响
- Author:
Lanyan ZHU
;
Ping CHEN
- Publication Type:Journal Article
- Keywords:
Celecoxib;
Lung cancer;
Cyclooxygenase-2;
Vascular endothelial growth factor
- From:
Chinese Journal of Practical Internal Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the selective cyclooxygenase-2 inhibitor celecoxib on the expression of PGE2 and VEGF induced by IL-1? in A549 cell lines.Methods A549 cells were incubated with IL-1? in the presence or absence of celecoxib.After indicated incubation time,cells were harvested to detect VEGFmRNA by reverse transcription-polymerase chain rection(RT-PCR),the medium was collected to determine the concentration of PGE2 and VEGF by enzyme-linked immunosorbent assay(ELISA).Results After Celecoxib intervention,the formation of PGE2 and VEGF expression decreased more than control group(IL-1? induced),the difference being significance(P
