Characters of HIV-1-specific CD8 T-cell responses in peripheral circulation of chronically HIV-1-infected children undergoing highly active antiretroviral therapy
- VernacularTitle:HAART治疗HIV-1慢性感染儿童外周血特异性CTL的反应特点
- Author:
Lei JIN
;
Qingxia ZHAO
;
Zheng ZHANG
- Publication Type:Journal Article
- Keywords:
T-lymphocytes,cytotoxic;
dendritic cells;
HIV;
child
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To characterize the properties of human immunodeficiency virus-1-(HIV-1)specific cytotoxic T lymphocyte(CTL)responses,and to analyze the factors which may affect CTL responses before and after treatment with highly active antiretroviral therapy(HAART)in children with chronic HIV-1 infection.Methods A total of 24 HIV-1-infected children were studied.Circulating CD4 T cell counts and HIV-1 RNA levels were routinely measured.Peripheral HIV-1-specific CTL frequency and the specific interferon-?-producing lymphocytes were determined by tetramer staining and enzyme-linked immunospot(ELISPOT)assay,respectively.Results It was found that HIV-1-specific CTL responses could be detected in vitro in more than 80% of Chinese children with HIV-1 infection.Circulating CTL frequency and IFN-?-producing lymphocyte counts were significantly reduced in HAART suppressed HIV-1 infected children in comparison with HAART failure children and HAART na?ve children.The duration of virus suppression after HAART was found to be inversely correlated with CTL frequency.Conclusion HIV-1-specific CTL response to virus was warranted by antigenic stimulation.The present work may elucidate the immune mechanisms influencing the outcome of HIV-1 carriers with treatment of HAART.
