Proteasome inhibitor MG-132 inhibits myocardial reperfusion injury in rats by suppressing expression of inflammatory factors
- VernacularTitle:蛋白酶体抑制剂MG-132下调炎症因子表达抑制大鼠心肌再灌注损伤
- Author:
Cuilian DAI
;
Kailiang LUO
- Publication Type:Journal Article
- Keywords:
reperfusion;
proteasome inhibitor;
inflammatory factor;
polymorphonuclear leucocyte;
myeloperoxidase
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of proteasome inhibitor MG-132 on inflammatory factors in rat model of myocardial ischemia/reperfusion. Methods Adult Sprague-Dawley rats were divided into 4 groups. I/R group: left anterior descending (LAD) coronary artery was ligated for 30 min, and then reperfused for 0, 1, 2, 6, 24 h; I/R+T group: MG-132 was used for treatment, and other procedures as I/R group; Sham operation group; Sham operation+T group. At 5 min before reperfusion, MG-132 (0.75 mg/kg) was given intravenously in I/R+T group and Sham operation+T group, and normal saline for I/R group and sham operation group. The changes of myocardial ultrastructure were checked, and the protein and mRNA levels of NF-?Bp65 and TNF-?, the number of PMN, the activity of MPO and survival of each group were detected. Results As compared with Sham operation group and Sham operation+T group, the protein and mRNA levels of NF-?Bp65 and TNF-?, the number of polymorphonuclear leucocytes (PMN) and the activity of myeloperoxidase (MPO) were significantly increased in I/R group (P
