Cloning of rat neuroglobulin gene and construction of its eukaryotic expression vector
- VernacularTitle:大鼠神经球蛋白基因的克隆及其真核表达载体构建
- Author:
Ji ZHU
;
Wenyuan TANG
- Publication Type:Journal Article
- Keywords:
neuroglobulin gene;
brain;
cloning;
expression vector
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone rat neuroglobulin (NGB) gene and construct its eukaryotic expression vector. Methods The total RNA was extracted from Wistar rat brain and the full length cDNA encoding NGB was obtained by RT-PCR. After the sequence was confirmed by sequencing and BLAST, it was inserted in the eukaryotic expression vector pCDNA3.1 (+), then the sequence and reading frame were confirmed by two restriction endonucleases and sequencing. Results The NGB gene was cloned with four bases mutated and its eukaryotic expression vector was constructed. Conclusion NGB expressed in Wistar rat brain. NGB gene was successfully cloned and inserted in eukaryotic expression vector.
