Study on Mechanism of Activities of Gastric Alcohol Dehydrogenases before and after Abetiuence of Alcohol.
- VernacularTitle:嗜酒前后胃乙醇脱氢酶活性变化机制研究
- Author:
Guoxiang WANG
;
Xuqing ZHU
;
Xin ZHANG
- Publication Type:Journal Article
- Keywords:
Alcohol dehydrogenase;
Hematoxylin eosin stain;
Enzyme electron microscope;
Stomach
- From:
Journal of Medical Research
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of activity change of gastric alcohol dehydrogenases(ADH).We investigated ADH before and after ingestion of alcohol on rats.Methods Thirty-nine rats were divided into two groups at random:model ones(24 cases) and control ones(15 cases).Model group of rats were infused into stomaches by alcohol,and control ones by distill water.Structures of cell and sub-cell in gastric mucous membrane were observed by enzyme electron microscope and hematoxylin eosin(HE) stain.Results It was shown that there were dropsical degeneration of chief cells and parietal cells of stomaches by HE stain,and the mitochondria of parietal cells and the rough-surfaced endoplasmic reticulum(rER) of chief cells were destroyed by enzyme electron microscope in model groups after two months of ethanol perfusion.There were not change in control group.Conclusions The mechanism of activity alterations of gastric ADH may result from dropsical degeneration of chief cells and parietal cells of stomach,which result in degeneration,solution and breakage of construction of rER in the chief cells and mitochondria in parietal cells.
