Dynamic changes of apoptotic neuron in hippocampus after pilocarpine induced status epilepticus in rats
- VernacularTitle:匹罗卡品致大鼠癫痫持续状态后海马神经元凋亡的动态观察
- Author:
Yingqi ZHANG
- Publication Type:Journal Article
- Keywords:
status epilepticus;
hippocampus;
caspase-3;
pilocarpine;
apoptosis
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To probe the occurrence of neuroal apoptosis in rats after status epilepticus(SE) and the relationship between neuron apoptosis and caspase-3 expression.Methods Rat model of SE was induced by intraperitoneal injection of 300 mg/kg pilocarpine in 20 male Wistar rats.Another 5 rats served as control.The apoptotic neuron was detected by TdT-mediated dUTP Nick End Labeling(TUNEL),and caspase-3 expression was observed by immunocytochemistry at 6~(th),48~(th),72~(th) h and 7~(th) d after SE induction.Results In control rats,TUNEL-positive neuron could not be found in hippocampus.The number of TUNEL-positive neuron began to increase at 6~(th) h after SE induction and peaked at 72~(th) h,began to decrease at 7~(th) d after SE induction.In control rats,there were few caspase-3 expression in hippocampus.The caspase-3 immunoreactivity began to increase at 6~(th) h after SE and peaked at 48~(th) h,especially in CA1 and CA3 region of hippocampus,began to decrease at 72~(th) h and dropped to normal level at 7~(th) d.The results indicated that the distribution of caspase-3 expression was consistent with the distribution of TUNEL-positive neuron.The peak of caspase-3 expression was earlier than that of TUNEL-positive neuron.Conclusion Neuron apoptosis participated in the process of delayed neuronal death in hippocampus after SE and was correlated with caspase-3 activation.
