Effect of inositol(1,4,5)-trisphosphate on intracellular calcium in rat detrusor cells
- VernacularTitle:激光共聚焦显微镜观察三磷酸肌醇对大鼠逼尿肌细胞内钙的影响
- Author:
Jun WANG
;
Bo SONG
;
Xiyu JIN
;
Enqing XIONG
- Publication Type:Journal Article
- Keywords:
inositol(1,4,5)-trisphosphat;
laser scan confocal microscopy;
overactive detrusor
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between IP_(3)/Ca~(2+)signal transduction process and the changes of intracellular calcium in detrusor cells in rats.Methods The model of overactive detrusor(OAD) was successfully established in female Wistar rats.Intracellular calcium fluorescence intensity of cultured detrusor cells in OAD rats and detrusor stability(DS) rats was measured by laser scanning confocal microscopy(LSCM).After pretreated by 10~(-5) mol/L IP_(3 )alone or with its inhibitor heparin,the intracellular calcium fluorescence intensity of cultured detrusor cells of DS rats in Ca~(2+)-contained and Ca~(2+)-free solutions was measured with LSCM.Results Intracellular calcium fluorescence intensity of cultured detrusor cells in OAD rats was stronger than that of DS rats(P
