Effect of D-amphetamine on apoptosis and GAP-43 expression of rat brain cells after focal cerebral ischemia
- VernacularTitle:右-苯丙胺对脑缺血大鼠细胞凋亡和生长相关蛋白43的作用
- Author:
Hongmei LI
;
Nong XIAO
;
Xiaoping ZHANG
- Publication Type:Journal Article
- Keywords:
brain ischemia;
growth-associated protein 43;
D-amphetamine;
apoptosis
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protection of D-amphetamine on rat brain after focal cerebral ischemia.Methods The unilateral middle cerebral artery occlusion(MCAO) models were established by using Koizumi's method.TUNEL was applied to detect quantitatively brain cell apoptosis at 1st,3rd and 6th week after operation.Immunohistochemical staining and RT-PCR were respectively used to detect the expression of growth-associated protein 43(GAP-43) and GAP-43 mRNA around ischemic area.Results Apoptosis of brain cells reduced evidently in the group treated with D-amphetamine.GAP-43 protein detection demonstrated statistically significant increase in immunoreaction product as determined by optical density measurements in D-amphetamine treated group compared with the group without any agent treatment.The same results appeared in RT-PCR product.Conclusion D-amphetamine can reduce brain cell apoptosis and promote GAP-43 expression.
