VEGF expression in choroid of streptozotocin-diabetic Wistar rats
- VernacularTitle:血管内皮生长因子在糖尿病大鼠脉络膜视网膜的表达
- Author:
Xiyuan ZHOU
- Publication Type:Journal Article
- Keywords:
diabetic retinopathy;
diabetic choroidopathy;
immunohistochemistry;
vessel endothelial growth factor
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression time and the position of VEGF in the choroid of diabetic rats and compare them with the VEGF expression in the choroid and retina of normal rats.Methods Healthy male Wistar rats were selected and randomly divided into diabetic group and normal group.Type 1 diabetes rat model was induced by being injected of large-dose streptozotocinum(STZ) into abdominal cavity.The retina and choroid of rats were obtained to detect the VEGF expression by immunohistochemistry at 1~(st),2~(nd),3~(rd),4~(th),5~(th) month after diabetes induction.Results VEGF expression in retina and choroid of rats was of obvious difference between normal group and one-month diabetic group.In two-month diabetic group,VEGF expression in choroid was positive(33.3%),while VEGF expression in retina was not significantly different with that of normal group.In three-month diabetic group,VEGF expression in choroid was positive(55.6%),and VEGF expression in inner nuclear layer and ganglion cell layer of retina was positive(33.3%).In four-month diabetic group,VEGF expression in choroid was positive(66.7%),and VEGF expression in inner limiting membrane,inner nuclear layer,outer nuclear layer and ganglion cell layer of retina was positive(77.8%).In five-month diabetic group,VEGF expression in choroid was positive(88.9%),and VEGF expression in the whole retina was positive(88.9%).The expression density of VEGF in choroid and retina gradually increased((P
