Studies on the toxicity of immunosuppressive agent to human islet cells in vitro
- VernacularTitle:免疫抑制剂对人胰岛细胞的毒性作用
- Author:
Zhen WANG
;
Bingyi SHI
;
Jiangping GAO
- Publication Type:Journal Article
- Keywords:
islets of Langerhans transplantation;
immunosuppressive agents;
toxic actions
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the direct deleterious effect of immunosuppressive agent to human islets, and to explore the suitable immunosuppressive protocol for human islet transplantation. Methods Human islet cells cultured in vitro were exposed to various therapeutic concentrations of four immunosuppressive agents for 72h respectively. Glucose-stimulating insulin secretion during incubation was measured using the ultrasensitive human insulin ELISA kit. The assessment of islet cell viability was studied with acridine orange (AO)-propidium iodide (PI). Results In vitro, low therapeutic concentrations had no deleterious effect on insulin secretion or viability of islet, but high concentration of the drugs was deleterious to ?-cell survival as well as insulin secretion. The effect of medium concentration varied significantly, and sirolimus tacrolimus demonstrated stronger deleterious effect on human islets than mycophenolic acid. Conclusion The direct deleterious effect of immunosuppressive agents to islet cells is dose-dependent, therefore immunosuppressive agent must be optimized after islet transplantation.
