The effect of recombinant human growth hormone on colorectal cancer cell lines radiosensitivity
- VernacularTitle:重组人生长激素对人结直肠癌细胞株放疗敏感性的影响
- Author:
Zhe XU
;
Lixia XU
;
Xiaoyu WU
;
Jieshou LI
- Publication Type:Journal Article
- Keywords:
Growth hormone;
Growth hormone receptor;
Colorectal cancer;
Radiation
- From:
Parenteral & Enteral Nutrition
1997;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of recombinant human growth hormone(rhGH) on colorectal cancer cell lines radiosensitivity.Methods:The GHR positive HCT-8 and GHR negative LOVO cell lines were used and each cell set was divided into 3 groups,which were radiation along,radiation + rhGH group and radiation + rhGH + GHRA(GHR neutralizing antibodies) group.The classical colony forming assay was performed to measure the post-radiation colorectal cancer cell proliferation as an indicator of radiosensitivity.Flow cytometry was used to detect the radiation induced apoptosis.Comet assay was performed to detect the radiation induced DNA damage.The radiation dose was 2 Gy,4 Gy,and 8 Gy respectively.The rhGH concentration used was 100 ng/mL while GHRA was 0.2 ug/mL.Results:The colony formation rate was significantly enhanced in HCT-8 cells pre-incubated with rhGH compared to the radiation group cells.Under the radiation of high dose(8 Gy),this effect was more obvious(52.1 2.9 vs 21.0 2.7,P
