Identification of interaction between HT036 and P311 by co-immunoprecipitation
- VernacularTitle:利用免疫共沉淀验证HT036与P311间的相互作用
- Author:
Shunzong YUAN
;
Xu PENG
;
Bing MA
;
Qinghong WANG
;
Shaoxuan YI
;
Weifeng HE
;
Xiwei CHEN
;
Xiaohong HU
;
Xiaorong ZHANG
;
Lina ZHOU
;
Gaoxing LUO
;
Ju WU
- Publication Type:Journal Article
- Keywords:
HT036;
P311;
expression vector construction;
co-immunoprecipitation;
protein-protein interaction
- From:Journal of Third Military Medical University
2003;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the interaction between HT036(hypothetical protein HT036)and P311 by co-immunoprecipitation.Methods HA-tagged fusion protein(HA-HT036)expression vector was constructed,identified and transfected into human embryo kidney 293(HEK293)cells alone or with Myc-tagged fusion protein(Myc-P311)expression vector pCMV-Myc-p311.The interaction between P311 and HT036 was detected by co-immunoprecipitation.Results Double restriction enzyme digestion showed that pCMV-HA-HT036 was constructed correctly.When Myc-P311 was immunoprecipitated by anti-Myc antibody,HA-HT036 was identified by Western blotting with anti-HA antibody from immunoprecipitated complex.Conclusion The recombinant vector pCMV-HA-HT036 was constructed successfully.The interaction between HT036 and P311 could be identified by co-immunoprecipitation after co-expression of pCMV-HA-HT036 and pCMV-Myc-p311.The result provides an important basis for further study of the intracellular signal transduction of P311.
