Protective effect of combined NGF and ganglioside GM_1 on hypoxic-ischemic brain injury of newborn rats
- VernacularTitle:神经生长因子和神经节苷脂对新生鼠缺氧缺血性脑损伤海马神经细胞的保护作用
- Author:
Wei HU
;
Congmin ZHAO
;
Shoupin GONG
- Publication Type:Journal Article
- Keywords:
hypoxic-ischemic brain injury;
nerve growth factor;
ganglioside GM_ 1;
apoptosis
- From:Journal of Third Military Medical University
2003;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the therapeutic effect of combined administration of nerve growth factor(NGF)and ganglioside GM_ 1 on the hypoxic-ischemic brain injury(HIBI)rat model and the relevant involving mechanisms.Methods A total of 120 7-day old rats were randomly divided into 5 groups:sham-operation group,HIBI group,NGF group,GM_ 1 group and NGF+GM_ 1 group.HE staining,immunohistochemical staining and TUNEL staining were employed respectively to study the morphological changes,bcl-2 and Bax expressions and apoptosis in the hippocampal neuron after treatment.Results Pathological changes were observed in the hippocampal neuron after HIBI.In NGF+GM_ 1 group,the level of Bcl-2 expression was increased significantly(P
