Protective effect of sodium and magnesium saline of fructose-1,6-diphosphate on B cells in pancreatic islet injured by streptozotocin
- VernacularTitle:果糖二磷酸钠镁对链脲佐菌素致胰岛B细胞损伤的保护作用
- Author:
Donghong PENG
;
Nen ZHANG
- Publication Type:Journal Article
- Keywords:
fructose-1,6-diphosphate;
streptozotocin;
islet of Langerhans;
cell injury;
cytoprotection
- From:Journal of Third Military Medical University
2003;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether sodium and magnesium saline of fructose-1,6-diphosphate(Na +-Mg 2+-FDP)protects B cells in islet of Langerhans injured by streptozotocin(STZ).Methods Healthy adult male Wistar rats were randomly divided into five groups:STZ group(receiving once 2% STZ in citric acid solution at dose of 60 mg/kg),blank control group(receiving citric acid solution),FDP group(treated as STZ group,plus Na +-Mg 2+-FDP solution twice a day at total dose of 500 mg?kg-1?d-1 for 15 d),protected group(receiving Na +-Mg 2+-FDP solution at 500 mg/kg first,then 2% STZ in citric acid solution at dose of 60 mg/kg once,Na +-Mg 2+-FDP solution twice a day at total dose of 500 mg?kg-1?d-1 for 15 d),treated group(48 h after receiving 2% STZ in citric acid solution at dose of 60 mg/kg,starting Na +-Mg 2+-FDP solution twice a day at total dose of 500 mg?kg-1?d-1 for 15 d).On the 15 th day after experiment starting,all rats were killed and the end of pancreatic gland was prepared for following experiments.Blood glucose by using glucose oxidase kit and serum insulin by radioimmunoassay was determined.The number of positive islets of which A,B and D cells expressed glucagons,insulin and somatostatin was counted by S-P immunohistochemistry.The morphology of islet of Langerhans stained by HE was observed under optical microscope.Statistical significance was determined by q test and ? 2 test,the related coefficient was determined by rectilinear correlation analysis(?=0.05).Results The blood glucose dropped and serum insulin rose in protected group,but the glucose in treated group and STZ group maintained at high level,and serum insulin maintained at low level.The blood glucose and insulin of the protected group showed significant differences with that of the treated group and STZ group respectively(P0.05).However,the expressions of insulin,gluca-gons and somatostatin were close to normal level in protected group,which were of statistical significance with that of STZ group and treated group(P
