Expression of TGF?_1 and CTGF mRNA in Liver of Rats with Nonalcoholic Fatty Hepatic Fibrosis Induced by Highly Fat-enriched Diet
- VernacularTitle:高脂饮食诱导的脂肪性肝纤维化大鼠肝组织TGF?_1和CTGFmRNA表达及其意义
- Author:
Guoqiang LOU
;
Hu WAN
;
Junping SHI
- Publication Type:Journal Article
- Keywords:
Nonalcoholic fatty liver disease;
Hepatic fibrosis;
Transforming growth factor ?_1;
Connective tissue growth factor;
Rats
- From:
Journal of Medical Research
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To reveal the expression and potential mechanisms of TGF?_1 and CTGF mRNA in liver of rats with nonalcoholic fatty hepatic fibrosis induced by highly fat-enriched diet.Methods The model group(10 rats) was fed with highly fat-enriched diet while the normal control group(10 rats) was raised by standard animal feeds for 24 weeks.Hepatic histopathological changes were evaluated,and RT-PCR method was used to assay TGF?_1 and CTGF mRNA expression levels in the liver.Results The analysis indicated that liver inflammation and fibrosis were apparently present in model rats.RT-PCR analysis revealed that the expression of TGF?_1 and CTGF in hepatic tissue were inreased in the model group as compared with the normal control group.Conclusions The rats model for hepatic fibrosis can be established by feeding with highly fat-enriched diet for 24 weeks.The expression of TGF?_1 and CTGF were enhanced in hepatic liver tissue of fatty hepatic fibrosis rats,and these changes might stimulate the fatty fibrosis.
