The Relationship Between Liver Histological Change and Expression of HBcAg in Peripheral Blood Dendritic Cells in Patients with Chronic Hepatitis B
- VernacularTitle:慢性乙型肝炎患者外周血树突状细胞内HBcAg存在与肝组织学改变的关系
- Author:
Yaoren HU
;
Zhiqiang XU
;
Xier TANG
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Chronic hepatitis B;
Pathogenesis
- From:
Journal of Medical Research
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between liver histological changes and expression of HBcAg in peripheral blood dendritic cells in patients with chronic hepatitis B.Methods The peripheral blood samples were obtained from 24 patients with chronic hepatitis B (CHB) and 8 healthy individuals.The peripheral blood mononuclear cells (PBMCs) were isolated.DCs were isolated from peripheral blood mononuclear cells and enriched by culturing with recombinant human interleukin 4(rhIL-4) and recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF),then DCs were harvested and the costimulators of CD80,CD86,CD40 and CD54 on DCs were tested by immunofluorescence flow cytometry.These costimulators were compared with liver histological changes.Results Percentage of CD40 and CD86 was significant lower than that of healthy donors(P
