The Expression of Cytoskeleton and Inflammatory Factor in Mice Kedney with the Unilateral Ureteral Obstruction
- VernacularTitle:输尿管结扎对小鼠肾皮质骨架蛋白与炎症因子表达的影响
- Author:
Yinghua ZHANG
;
Ruchun YANG
;
Yi LIN
;
Jun WANG
;
Xiaoling ZHU
- Publication Type:Journal Article
- Keywords:
Unilateral ureteral obstruction;
Cytoskeleton;
Intercellular adhesion molecule 1;
Monocyte chemoattractant protein 1
- From:
Journal of Medical Research
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate renal interstitial fibrosis and the expression of inflammatory factor in mice kedney with the Unilateral Ureteral Obstruction(UUO).Methods Mice were randomly assigned to shame operation group (shame proup ), UUO group. The protein expression of?-SMA and Vimentin were assessed by immunohistochemistry and the protein expression of ICAM-1 were assessed by Western-blotting; MCP-1 mRNA were detected by using reverse transcription-polymerase chain reaction analyses(RT-PCR). Results In comparison with the shame group, the mRNA expression, protein levels of ?-SMA, Vimentin of UUO mice groups increased significantly. At the same timre, the protein expression ICAM-1 and the mRNA expression MCP-1 in UUO mice significantly higher than normal group.Conclusions Unilateral ureteral obstruction could induce the transdifferentiation of renal interstitial cells. It may partially related to the high expression of ICAM-1and MCP-1.
