THE PROTECTIVE EFFECT OF EXOGENOUS NUCLEOTIDES ON LIPOPOLYSACCHARIDE IMMUNOSTIMULATION IN MICE AND ITS MECHANISM
- VernacularTitle:外源核苷酸对脂多糖刺激小鼠的保护作用及其机制研究
- Author:
Shiying LI
;
Yonghui SHI
;
Guowei LE
- Publication Type:Journal Article
- Keywords:
nucleotides;
lipopolysaccharide(LPS);
oxidation;
inflammation
- From:
Acta Nutrimenta Sinica
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the protective effects of exogenous nucleotides on lipopolysaccharide(LPS)immunnostimulation and its mechanism.Method:Forty healthy Kunming mice were randomly divided into five groups:control group,nucleotides(NT)groups(4h,18h),nucleotides free(NF)groups(4h,18h).Control group and NF groups were fed with nucleotide-free diet.NT groups were fed with nucleotide-supplemented diet(0.25% nucleotides).On D 15,mice were lavaged with physiological saline(control)or LPS,and were killed 4 or 18 h later.Serum,liver,small intestine,and peritoneal macrophage were sampled in germfree state.Results:Hepatic Na+K+-ATPase,intestinal superoxide dismutase(SOD),serum total anti-oxidation ability,peritoneal macrophage-produced interleukin 10(IL-10)were increased,and intestinal malonaldehyde(MDA),serum alanine aminotransferase(ALT),intestinal myeloperoxidase(MPO),peritoneal macrophage-produced interleukin 1(IL-1)were decreased with nucleotides supplement.Conclusion:Exogenous nucleotides can help to maintain oxidation-antioxidation and inflammation-antiinflammation balance,and protect mice from injury under LPS immunostimulation.
