INHIBITION OF OXIDIZED LIPOPROTEINS INDUCED APOPTOSIS OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS BY TEA POLYPHENOLS
- VernacularTitle:茶多酚对ox-LDL诱导人脐静脉内皮细胞凋亡的抑制作用
- Author:
Chunxia QIAO
;
Guifa XU
;
Xiulan ZHAO
- Publication Type:Journal Article
- Keywords:
tea polyphenols;
oxidized low density lipoprotein;
human umbilical vein endothelial cells;
apoptosis;
Bcl-2;
Bax;
caspase-3
- From:
Acta Nutrimenta Sinica
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the inhibition and mechanism of tea polyphenols (TP) on apoptosis induced by oxidized low density lipoprotein (ox-LDL) in human umbilical vein endothelial cells (HUVEC). Method: HUVEC cells were administered with TP (25 ?g/ml), ox-LDL (200 ?g/ml)+TP (25 ?g/ml), ox-LDL (200 ?g/ml) respectively, cells with equal volume of solvent as control. Cell viability was determined by MTT assay, apoptosis by acridine orange fluorescence dyeing, and expressions of Bcl-2、Bax and caspase-3 were analyzed by Western blotting. Results: Ox-LDL could inhibit HUVEC cell proliferation. After treated with both TP and ox-LDL , the cell proliferation increased obviously and showed significant difference from the cells treated with ox-LDL only (P
