EFFECTS OF (-)EPIGALLOCATECHIN GALLATE AND BLACK TEA POLYPHENOLS ON LIPID METABOLISM RELATED GENE EXPRESSION OF HepG_2 CELLS
- VernacularTitle:EGCG和红茶多酚对HepG_2细胞脂质代谢相关基因表达的影响
- Author:
Huijun DENG
;
Yi LI
;
Juan YI
;
Jin CAO
- Publication Type:Journal Article
- Keywords:
(-)epigallocatechin gallate(EGCG);
black tea polyphenols;
HepG2 cells;
gene expression profile
- From:
Acta Nutrimenta Sinica
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of (-) epigallocatechin gallate (EGCG) and black tea polyphenols on the lipid metabolism related gene expression profile of human hepatocellular carcinoma HepG2 cells. Method: The total RNA was isolated from HepG2 cells treated with EGCG (5? mol/L), black tea polyphenols (5?g/ml) or 0.1%DMSO (control) for 8 h and was hybridized to Human 14k cDNA microarray for gene expression profile analysis. Real-time RT-PCR was conducted to confirm microarray data. Results: A total of 13 and 29 genes related to lipid metabolism showed differential change after EGCG or black tea polphenols treatment respectively, of which six genes showed consistent expression. The results of real-time PCR were consistent with the microarray data. Conclusion: The mechanism(s) by which EGCG and black tea polyphenols exerts its effects on lipid metabolism are comprehensive. The novel target identified in this study may provide new evidence for further investigation in the hypolipidemic effects of tea polyphenols.
