Inhibitory effect of bicyclol on iNOS expression and NF-?B activation degradation in marcrophages induced by lipopolysaccharides
- VernacularTitle:双环醇对脂多糖诱导巨噬细胞iNOS蛋白的表达和NF-?B活化的抑制作用
- Author:
Min LI
;
Gengtao LIU
- Publication Type:Journal Article
- Keywords:
bicyclol;
macrophage;
inflammation;
TNF-?;
iNOS;
NF-?B
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim Inflammation is a common characteristic of liver injury induced by hepatitis virus or other factors.The purpose of this paper was to study the modulating effect of bicyclol,a novel anti-hepatitis drug,on related inflammatory molecules.Methods Various non-cytotoxic concentrations of bicyclol were cultured with macrophage RAW264.7 line or mouse peritoneal macrophages for 1 h,and then added adequate lipopolysaccharide(LPS)to activate macrophages.NO and TNF-? levels in the supernatant of the culture medium were determined with Griess reagent and L929 cell bioassay,respectively.The iNOS expression and NF-?B activation were detected by Western blot method.Results Pretreatment of 0.1～0.5 mmol?L-1 bicyclol significantly inhibited NO release and TNF-? secretion from RAW264.7 cell line and mouse peritoneal macrophages induced by 1 mg?L-1 LPS in concentration dependent manners and 0.5 mmol?L-1 bicyclol also down-regulated LPS(1 mg?L-1)-induced iNOS expression and NF-?B activation in the cells.Conclusion Bicyclol has suppressive effect on related inflammatory molecules iNOS expression and NF-?B activation,indicating that bicyclol possesses anti-inflammatory property.
