Regulatory effect of catalpol from Radix Rehmanniae on M_2 receptor density in M_2 receptor transfected CHO cells
- VernacularTitle:地黄活性成分梓醇对转基因CHO细胞M_2受体的调节作用
- Author:
Jinhong WANG
;
Qixiang SUN
;
Zongqin XIA
;
Yaer HU
- Publication Type:Journal Article
- Keywords:
catalpol,CHOm2 cells,M_2 receptor density
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of catalpol from Radix Rehmanniae on M_2 receptor density in CHO cells transfected with gene of M_2 receptors(CHO m2).Methods Cultured CHOm2 cells were randomly divided into 4 groups:three concentrations of catalpol :10-6、10-5、10-4 mol?L-1,and saline control.After addition of catalpol and saline for 72 h,M_2 Receptor density was measured by single point 3H-NMS binding assay,the content of protein was measured with Lowry's method.Competitive binding assay using the binding system of 3H-NMS was performed to address the question about whether catalpol could occupy the M receptor binding site.Addition of catapol to brain homogenate and measuring the enzyme activity with the Ellman's colorimetric method were performed to address the question about whether catalpol could in-hibit acetylcholinesterase activity.Results Catalpol can elevate the M_2 receptor density in CHO m2 cells significantly at the doses of 10-5、10-4 mol?L-1(P
