Effect and Mechanisms of Isoflurane on energy metabolism after Ischemia in Isolated Rat Hearts [WT5BZ]
- VernacularTitle:异氟烷对离体大鼠心脏缺血后能量代谢的影响及其机制
- Author:
Pengcheng XU
;
Nuo XU
;
Yiqiao WANG
;
Jianwei FAN
- Publication Type:Journal Article
- Keywords:
isoflurane;
protein kinase C;
metabolism;
myocardial ischemia
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To determine the effects and mechanisms of isoflurane on energy metabolism during ischemia and reperfusion in isolated rat hserts.Methods The rat Langendorff model was used,and isolated per-fused rat hearts were separated into untreated,isoflurane,chelerythrine(PKC inhibitor)plus isoflurane,and chelerythrine groups.All the hearts were subjected to treatment before ischemia,followed by 30 min of ischemia and 60 min of reperfusion.Hemodynamic variables were recorded,and metabolites were measured by high-performance liquid chromatography,and analyzed subcellular localization of PKC isoforms by Western blot analysis.Results The recovery of left ventricular developed pressure after ischemia was(33?8)%,(56?9)%,and(30?5)% in the untreated,isoflurane,and isoflurane with chelerythrine groups respectively.Compared with the untreated hearts,isoflurane significantly improved the recovery of left ventricular developed pressure(P
