Protective effect of Icariin on primary cultures of neonatal rat cardiomyocytes treated with isoproterenol-induced injury
- VernacularTitle:淫羊藿苷对异丙肾上腺素所致乳鼠心肌细胞损伤的保护作用
- Author:
Hua LIU
;
Hui JI
;
Chaoying ZHANG
- Publication Type:Journal Article
- Keywords:
Icariin;
isoproterenol;
neonatal rat cardiomyocytes;
mitochondrial membrane potential;
apoptotsis
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protection effect of icariin on isoproterenol induced neonatal rat cardiomyocytes injury.Methods Isolation of cardiomyocytes from Sprague-Dawley rats aging 1～3 d were performed.Cardiomyocytes were grown in DMEM culture medium containing 15% fetal bovine serum.The cell density was adjusted to 1?105～5?105 cells?L-1,and the suspension was pipetted into each well of culture plates.Cells were incubated at 37 ℃ in 5% CO_2 atmosphere for 72 h.Cultured rat cardiomyocytes were divided into different groups:control,0.5 mmol?L-1 isoproterenol(model),and therapeutic groups with 0.1,1 and 10 ?mol?L-1 icariin.Cell viability was analyzed using MTT assay.The potential of mitochondrial membrane was determined by laser scanning confocal microscope.Apoptotic cells were detected by using flow cytometric analysis.Results Compared with the control group,the cell activity was lower in model group(P
