Effects of globular adiponectin,glucose and free fatty acid on AMPK and ACC phosphorylation in INS-1 ? cells
- VernacularTitle:球形脂联素、葡萄糖和游离脂肪酸对胰岛?细胞单磷酸腺苷激活的蛋白激酶和乙酰辅酶A羧化酶磷酸化的影响
- Author:
Yu TONG
- Publication Type:Journal Article
- Keywords:
Islets of Langerhans;
Glucose;
Fatty acids,nonesterified;
Adiponectin
- From:
Journal of Peking University(Health Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate effects of glucose and free fatty acid at different concentrations on phosphorylation of adenosine-5′-monophosphate activated protein kinase(AMPK) and acetyl CoA carboxylase(ACC) in INS-1 cells,and effects of globular adiponectin on phosphorylation of AMPK and ACC.Methods: INS-1 cells were cultured and treated with 5 mmol/L glucose or 0.25 mmol/L free fatty acids,and time courses and dose responses of different dosages of glucose and fatty acid on phosphorylation of AMPK and ACC were measured.We measured the effects of the pharmacological AMPK activator AICAR(5-aminoimidazole-4-carboxamide-riboside) and globular adiponectin on phosphorylation of AMPK and ACC.Results: Glucose and fatty acid at different concentrations inhibited the phosphorylation of AMPK and ACC at the end of 60 min,but AICAR increased the phosphorylation of AMPK and ACC significantly,while 2.5 mg/L globular adiponectin increased the phosphorylation of AMPK and ACC by 23%(P
