Identification of low-molecular weight prostate-specific antigen(PSA) and lactoferrin in the prostatic secretion of benign prostatic hyperplasia
- VernacularTitle:良性前列腺增生的前列腺液中低相对分子质量前列腺特异抗原及乳铁蛋白的表达
- Author:
Kexin XU
;
Xiaofeng WANG
- Publication Type:Journal Article
- Keywords:
Prostate-specific antigen;
Lactoferrin;
Prostatic hyperplasia
- From:
Journal of Peking University(Health Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of low-molecular-weight PSA(lw-PSA) and lactoferrin in the expressed prostatic secretion(EPS) from both benign prostatic hyperplasia(BPH) and normal prostate.Methods: Forty human EPS samples obtained from 20 BPH patients and 20 normal males were subjected to two-dimensional gel electrophoresis(2-DE).Mass spectrometry was performed to confirm the nature of the secreted proteins in EPS.Results: One uniquely expressed protein in BPH was detected and mass spectrometry determined its nature as lw-PSA(molecular weight 10?10~3,pI 8.5-(9.3)).More importantly,Western blotting analysis also revealed that lw-PSA detected in BPH-EPS,but was undetectable in BPH-free EPS.In addition,up-regulation of Lactoferrin(molecular weight 35?10~3,pI 7-7.5) in BPHEPS,as compared with BPH-free EPS,was also observed.More interestingly,lactoferrin was absent in prostate cancer tissues.Conclusion: Our results indicate lw-PSA may be produced specifically by BPH epithelium and it has a potential to be used as a specific biological marker for the diagnosis of BPH.In addition,benign prostatic epithelium can produce more lactoferrin while prostate cancer tissues go without its lactoferrin secretion.
