Detection of aminoglycoside acetyltransferases in Acinetobacter baumannii by multiplex PCR
- VernacularTitle:多重PCR检测鲍曼不动杆菌氨基糖苷乙酰转移酶相关基因
- Author:
Ding LIU
;
Ping CHEN
;
Yuhong LI
;
Cheng YANG
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
PCR;
aminoglycoside resistant
- From:Journal of Third Military Medical University
2003;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the distribution of aminoglycoside acetyltransferases (aac) gene in Acinetobacter baumannii, and to establish a molecular biology method to detect the drug-resistance of the bacteria. Methods The aac genes of 42 isolated Acinetobacter baumannii were analyzed by drug susceptibility and multiplex PCR assay. Results The isolated bacteria were more resistant to Gentamicin (57%) and less resistant to Amikacin (29%). As to the bacteria resistant to at least one of the following aminoglycosides, Gentamicin, Tobramycin, Amikacin, aminoglycoside resistant genes were detected in all of pathogens except 2 special. Among them 6 strains with aac(6′)-Ih, 20 strains with aac(3)-Ia, 14 strains with aac(6′)-Ib and 14 strains were found to have both aac(6′)-Ib and aac(3)-Ia. Conclusion Acinetobacter baumannii isolated in this district are easily resistant to Gentamicin, and it is most caused by the enzyme encoded by aac(3)-Ia and aac(6′)-Ib.
