Effects of TNF alpha on expression of SREBP-1c mRNA and triglyceride contents in cultured steatosis hepatocytes
- VernacularTitle:TNF-?对脂肪变性肝细胞SREBP-1c的表达及甘油三酯含量的影响
- Author:
Linhui YANG
;
Dongfeng CHEN
- Publication Type:Journal Article
- Keywords:
TNF alpha;
TNF alpha antibody;
steatosis hepatocyte;
sterol-regulatory element binding protein-1c;
triglyceride
- From:Journal of Third Military Medical University
2003;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of TNF alpha on the expression of sterol-regulatory element binding protein-1c, SREBP-1c mRNA and the content of triglyceride (TG) in the models of cultured steatosis hepatocytes. Methods Steatosis models of hepatocytes were established by adding oleic acid to the growing L-02 cells, and then cultured in present with TNF alpha or its antibody. The expressions of SREBP-1c and fatty acid synthetase (FAS) mRNA were measured with RT-PCR, lipid droplets in the hepatocytes were observed with oil red staining and the TG contents in hepatocytes were measured with analyzed kit. Results SREBP-1c mRNA was upregulated in the TNF alpha treatment group in comparison with the normal control group(P
