Effect of diabetic neuropathy on renal 1-? hydroxylase and bone mineral density in old type 2 diabetic rats
- VernacularTitle:老龄大鼠2型糖尿病肾病对肾1-?羟化酶及骨质密度影响的实验研究
- Author:
Changquan HUANG
;
Gang CHENG
;
Fen YE
- Publication Type:Journal Article
- Keywords:
old;
type 2 diabetic rats;
renal 1-alpha hydroxylase;
1,25(OH)_ 2 D_ 3;
bone mineral density
- From:Journal of Third Military Medical University
2003;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of renal 1-alpha hydroxylase and its effect on bone mineral density in the old rats with type 2 diabetic nephropathy. Methods Forty Wistar rats of 18 months old were randomly divided into normal control (N), diabetes (D), diabetes treated with vitamin D_ 3 (T1) and diabetes treated with 1-?(OH)D_ 3 (T2) groups, respectively. Ten rats in each group. Dual energy X-ray absorption (DEXA) was used to determine bone mineral density (BMD) of lumbar spines and femoral bone. 24 h urinary protein excretion, serum 25(OH)D_ 3 and 1,25(OH)_ 2 D_ 3 were measured by radioimmunoassay. Results Compared with controls, 24 h urinary protein excretion increased remarkably D, T1, T2 groups, while BMD greatly decreased, much lower in D group and T1 group than T2 group (P0.05). The level of 1,25(OH)_ 2 D_ 3 in N group was the same to T2 group, but higher than D, T1 groups (P
